5 Simple Techniques For 가슴수술
5 Simple Techniques For 가슴수술
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Though we haven't examined PneumaCult™ media to be used with murine epithelial cells, you will find publications that report this application.
RNPs are shipped to CD34+ cells through the CellPore™ Transfection System. Write-up-transfection, cells are returned to tradition and subsequently analyzed for enhancing efficiency by means of recognized approaches as permitted from the experimental layout, such as circulation cytometry or ArciTect™ T7 Endonuclease I Package (Catalog #76022). Edited progenitor cell purpose can be measured by in vitro colony-forming device (CFU) assays or cells can be more cultured for lineage-precise differentiation using customized StemSpan™ media and dietary supplements.
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This item is suitable for use in the next investigation area(s) as 줄기세포 지방이식 Component of the highlighted workflow phase(s). Examine these workflows to learn more in regards to the other goods we offer to aid Every single research location. Investigate Region Workflow Phases
For ideal final results, the total quantity of cargo extra shouldn't exceed ten% 자가지방이식 in the response quantity. Decreasing the reaction quantity to under eighty µL may possibly cause reduce enhancing efficiencies and cell recoveries.
There are lots of commercially out there sources for primary airway epithelial cells. Now we have examined HAECs from two vendors, Lonza and Epithelix — equally have resulted in prosperous ALI cultures.
If greater clumps remain noticeable in the answer, return the tube to 37°C for an additional two minutes and repeat the procedure until eventually organoids have totally damaged into single cells.
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Generally, HSAECs cultured in PneumaCult™-ALI-S Medium will 지방흡입 variety a completely differentiated cuboidal epithelium right after 4 to 5 weeks of society. Some donor variability could be expected.
Use cell counts to individual the sample into two tubes – “unstained” and “with viability 가슴수술 dye”.
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The next fluorochrome-conjugated antibodies and dyes are advised to facilitate analysis of gene-edited CD34+ HSPCs: